Expression of Mst89B and CG31287 is Needed for Effective Sperm Storage and Egg Fertilization in Drosophila.

摘要: In Drosophila, male reproductive fitness can be affected by any number of processes, ranging from development gametes, transfer to and storage mature sperm within the female organs, utilization for fertilization. We have previously identified 89B cytogenetic map position D. melanogaster as a hub genes that effect paternity success when disturbed. Here, we used RNA interference test 11 are highly expressed in testes located region their role competition fecundity expression is perturbed. Testes-specific knockdown (KD) bor CSN5 resulted complete sterility, whereas KD CG31287, Manf Mst89B, showed breakdown competitive second mate (P2 < 0.5) reduced single matings. The low explained significant reduction amount produced. Mst89B CG31287 does not affect production, into bursa or 30 min after mating. Instead, observed 24 h Egg hatchability mating also drastically females mated males, this parallels decrease fecundity. show normal germ-line needed effective usage egg