Detection of Helicobacter pylori gene expression in human gastric mucosa.

摘要: Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression subject to artificial conditions which may not accurately reflect host tissues. Gastric antral body biopsy specimens serum for anti-H. pylori immunoglobulin G serology were obtained from 42 patients. Biopsy studied histology, culture, reverse transcription PCR (RT-PCR). Oligonucleotide primers specific H. (16S rRNA, ureA, cagA) used detect bacterial mRNA gastric specimens. was performed on DNA corresponding isolates genomic 16S cagA. Of patients whom clinical obtained, 25 infected basis both histology or culture (i.e., tissue positive); 13 negative serology, culture; 4 positive only. RT-PCR rRNA detected 24 tissue-positive 0 17 tissue-negative (P < 0.001). ureA 16 CagA 14 group group. presence gene correlated absolutely tissue. These results indicate that a sensitive method detection genes human Detection vivo this approach contribute improving diagnosis understanding pathogenesis infections.