作者: A Thorfve , E Svala , H Brisby , P Thomsen , A Lindahl
DOI: 10.4172/2329-8820.1000151
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摘要: Background: Human mesenchymal stem cells (hMSCs) have the potential to differentiate at least into adipocytes, chondrocytes and osteoblasts. The differentiation capacity can be modulated by drugs, or chemical substances that affect diverse mechanisms essential for e.g. bone formation. aim of this study was investigate osteoinductive interleukin-6 (IL-6) inhibitor Madindoline A (MadA) its relation bone-inducing Wnt signaling pathways. Methods: After stimulation with MadA hMSC from 4 donors (aged 13-33 years) in an vitro culture, alkaline phosphatase (ALP) activity extracellular matrix (ECM) mineralization hMSCs were quantified calcification visualized von Kossa staining. expression bone- related markers further studied gene protein levels. In addition, non-canonical Wnt5a ligand added as a positive control, effect on IL-6 STAT3 phosphorylation evaluated. Results: Stimulation induced increased ECM upregulated genes RUNX2, COL1A1 Osteocalcin, although large donor-to-donor differences observed. Further, affected both canonical pathways displayed superior osteoinducing property compared some cases. Conclusion: summary, all higher reduced after stimulation. present results provide first time indications osteoinduction MadA.