Effects of dietary sphingomyelin on central nervous system myelination in developing rats.

摘要: Human milk contains sphingomyelin (SM) as a major component of the phospholipid fraction. Galactosylceramide (cerebroside), metabolite sphingolipids, increases along with CNS myelination, and is generally considered universal marker myelination in all vertebrates. l-Cycloserine (LCS) an inhibitor serine palmitoyltransferase (SPT), rate-limiting enzyme for sphingolipid biosynthesis that reported to show increased activity development rat CNS. The present study examined effects dietary SM on during LCS-treated rats. From 8 d after birth, Wistar pups received daily s.c. injection (100 mg/kg) LCS. 17 animals were fed 810 mg/100g bovine SM-supplemented diet (SM-LCS group) or nonsupplemented (LCS group). At 28 killed subjected biochemical morphometric analyses. myelin dry weight, total lipid content, cerebroside content significantly lower SM-LCS LCS groups than group not treated (the non-LCS However, these levels higher group. Morphometric analysis optic nerve revealed axon diameter, fiber thickness, g value (used compare relative thickness sheaths around fibers different diameter) other groups, but similar groups. These findings suggest contributes developing rats experimental inhibition activity.