Method for isolating preantral follicles from mare ovaries.

摘要: The aims of this study were to evaluate the use collagenase treatment isolate preantral follicles from mare ovaries and assess effect on follicular morphology. Intact chopped into pieces, incubated individually with 1, 3 or 5 mg (type 1A) ml(-1) in a shaking waterbath at 37 degrees C for up 2 h passed through series stainless steel filters pore size 50-300 microm remove large clumps stromal cells. samples prepared histological analysis sections examined by light microscopy. Isolated oocytes measured quality was assessed microscopic examination. Very few intact isolated after 30 min incubation ml(-l). After 60 90 incubations, between eight 71 ml(-1), whereas very 1 ml(-1). number either not significantly different. decreased increasing time no observed incubation. Preantral 60-300 diameter Most 90-150 diameter. This indicates that equine can be using does have deleterious follicle morphology when used appropriate concentration minimum period. However, oocyte viability remain determined.