Analysis of genetic diversity in Rose’s mountain toadlet (Capensibufo rosei) using novel microsatellite markers

摘要: AbstractOn the Cape Peninsula, Capensibufo rosei is known from only two isolated breeding populations within Table Mountain National Park. Because of its declining state, there an urgent need to understand genetic diversity, population structure and patterns movement this species. To do this, 15 microsatellite primer pairs were designed, optimised tested. Successful loci screened for null alleles genotyping errors then analysed, specifically noting number alleles, allelic size range, observed expected heterozygosities, deviations Hardy–Weinberg equilibrium (HWE) linkage disequilibria. Bottleneck tests analyses molecular variance (AMOVA) also conducted gain insight into each population’s structure. Eleven produced unambiguous polymerase chain reactions (PCR) products scoreable bands, which found be polymorphic across both populations. Deviations HWE detected owing presence and...