Sarcoplasmic reticulum Ca2+ pump mRNA stability in cardiac and smooth muscle: role of poly A+ tail length
作者： Lin Nie , Islam Khan , Christine M Misquitta , Ashok K Grover
DOI: 10.1016/J.CECA.2003.12.001
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摘要: Abstract Transcripts of the sarco/endoplasmic reticulum Ca2+ pump SERCA2 are alternatively spliced to produce SERCA2a (expressed in left ventricular muscle (LVM)) and SERCA2b (in stomach smooth (SSM)) mRNA that use different polyadenylation sequences. LVM is more stable than SSM. Here, we report poly A+ tail length (32±2 bases) longer SSM (20±1). In vitro decay assays, 3′-region or when longer. However, similar for SERCA2b, RNA stable. Thus, may contribute to, but does not appear be sole determinant of, greater stability
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