Glucosyltransferase CsUGT78A14 Regulates Flavonols Accumulation and Reactive Oxygen Species Scavenging in Response to Cold Stress in Camellia sinensis.

摘要: Glycosyltransferases (UGTs) play diverse roles in cellular metabolism by altering regulatory metabolites activities. However, the physiological of most members UGTs crops response to abiotic stresses are unknown. We have identified a novel glycosyltransferase CsUGT78A14 tea crops, an important economic whose expression is strongly induced cold stress. Biochemical analyses confirmed that CsUGT78A14-1 showed highest activity toward kaempferol and involved biosynthesis kaempferol-diglucoside, whereas product CsUGT78A14-2, which differs from single amino acid, was as 3-O-glucoside. The accumulation monoglucosides diglucosides consistent with levels stress, well different tissues genotypes plants. Down-regulation resulted reduced flavonols, reactive oxygen species (ROS) scavenging capacity finally plant stress tolerance under antioxidant flavonols aglycon enhanced glucosylation catalyzed CsUGT78A14. results demonstrate plays critical role increasing ROS capacity, providing insights into biological flavonoids