A Developmental Stage-Specific Promoter Directs Germline Transcription of DβJβ Gene Segments in Precursor T Lymphocytes
作者： Eugene M. Oltz , Michael L. Sikes , Jing Song , Rey J. Gomez
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摘要: The tissue- and stage-specific assembly of Ag receptor genes is regulated by transcriptional control elements positioned within Ig TCR loci. To further understand the role cis -acting in these regulatory mechanisms, we have characterized a promoter that drives germline expression TCRβ gene segments vivo. activity this promoter, termed PDβ, restricted to highly conserved 400-bp region located directly upstream from Dβ1-coding sequences. Maximal PDβ requires TATA element situated Dβ1 recombination signal sequences consensus binding sites for ubiquitous SP1 T cell-specific GATA-3 transcription factors. When linked active enhancer elements, directs most cell types; however, (Eβ) stimulates function specifically precursor lymphocytes. These findings suggest PDβ/Eβ interactions may contribute differential regulation regions locus during thymocyte development.
jimmunol.org 参考文章(43)
SH Orkin, GATA-binding transcription factors in hematopoietic cells Blood. ,vol. 80, pp. 575- 581 ,(1992) , 10.1182/BLOOD.V80.3.575.BLOODJOURNAL803575
L H Glimcher, M Boothby, H C Liou, Differences in DNA sequence specificity among MHC class II X box binding proteins. Journal of Immunology. ,vol. 142, pp. 1005- 1014 ,(1989)
R P Perry, G G Lennon, The temporal order of appearance of transcripts from unrearranged and rearranged Ig genes in murine fetal liver. Journal of Immunology. ,vol. 144, pp. 1983- 1987 ,(1990)
M. Serwe, F. Sablitzky, V(D)J recombination in B cells is impaired but not blocked by targeted deletion of the immunoglobulin heavy chain intron enhancer. The EMBO Journal. ,vol. 12, pp. 2321- 2327 ,(1993) , 10.1002/J.1460-2075.1993.TB05886.X
A J Henderson, S McDougall, J Leiden, K L Calame, GATA elements are necessary for the activity and tissue specificity of the T-cell receptor beta-chain transcriptional enhancer. Molecular and Cellular Biology. ,vol. 14, pp. 4286- 4294 ,(1994) , 10.1128/MCB.14.6.4286
P. Ferrier, B. Krippl, T.K. Blackwell, A.J. Furley, H. Suh, A. Winoto, W.D. Cook, L. Hood, F. Costantini, F.W. Alt, Separate elements control DJ and VDJ rearrangement in a transgenic recombination substrate. The EMBO Journal. ,vol. 9, pp. 117- 125 ,(1990) , 10.1002/J.1460-2075.1990.TB08087.X
J A Brockman, D C Scherer, T A McKinsey, S M Hall, X Qi, W Y Lee, D W Ballard, Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation. Molecular and Cellular Biology. ,vol. 15, pp. 2809- 2818 ,(1995) , 10.1128/MCB.15.5.2809
M M Davis, N E Lee, T cell receptor beta-chain genes in BW5147 and other AKR tumors. Deletion order of murine V beta gene segments and possible 5' regulatory regions. Journal of Immunology. ,vol. 140, pp. 1665- 1675 ,(1988)
L J Ko, M Yamamoto, M W Leonard, K M George, P Ting, J D Engel, Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Molecular and Cellular Biology. ,vol. 11, pp. 2778- 2784 ,(1991) , 10.1128/MCB.11.5.2778
M. Raducha, H. Harris, T. Kadesch, P. Henthorn, P. Zervos, Expression of a human placental alkaline phosphatase gene in transfected cells: use as a reporter for studies of gene expression Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 6342- 6346 ,(1988) , 10.1073/PNAS.85.17.6342