Amiloride: a molecular probe of sodium transport in tissues and cells

摘要: The potassium-sparing diuretic amiloride has proven to be a useful pharmacological tool for elucidating the molecular basis and physiological regulation of facilitated sodium entry in tissues cells. There are two general classes Na+ transport mechanisms which sensitive this drug: 1) conductive pathway found electrically high resistance epithelia 2) Na+-H+ electroneutral exchange system certain leaky such as renal proximal tubule. This latter is also many different cellular preparations seems function cell proliferation differentiation, volume regulation, intracellular pH regulation. In these cells, becomes operational usually after some external stimuli. Much higher concentrations required inhibit than those pathway. drug most potent specific inhibitor date thus affords opportunity used label isolation moieties.