Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.

摘要: During infection pathogens secrete small molecules, termed effectors, to manipulate and control the interaction with their specific hosts. Both pathogen plant are under high selective pressure rapidly adapt co-evolve in what is usually referred as molecular arms race. Components of host’s immune system form a network that processes information about molecules foreign origin damage-associated signals, integrating them developmental abiotic cues plant’s responses. case nucleotide-binding leucine-rich repeat receptors receptor kinases networks have been extensively characterized. However, little known on whether pathogenic effectors complexes overcome immunity promote disease. Ustilago maydis, biotrophic fungal infects maize plants, produces target hubs host cell. Here we assess capability U. maydis effector candidates interact each other, which may play crucial role during process. Using systematic yeast-two-hybrid approach based preliminary pooled screen, selected 63 putative for one-on-one matings library nearly 300 candidates. We found 126 these interacted either themselves or other predicted effectors. Although functional relevance observed interactions remains elusive, propose abundance complex formation between adds an additional level complexity research should be taken into consideration when studying evolution function. Based this fundamental finding, suggest various scenarios could evolutionarily drive stabilization interactome.