Molecular characterization of a carboxy-terminal eukaryotic-cell-binding domain of intimin from enteropathogenic Escherichia coli.
作者： G Frankel , D C Candy , E Fabiani , J Adu-Bobie , S Gil
DOI: 10.1128/IAI.63.11.4323-4328.1995
关键词:
摘要: A eukaryotic cell-binding domain from the intimin (Int) polypeptide of enteropathogenic Escherichia coli O127 (EPEC) was investigated. Derivatives carboxy-terminal 280-amino-acid domains Int (Int-EPEC280) and homolog invasin (Inv) Yersinia pseudotuberculosis (InvYP280) were fused to E. maltose-binding protein (MBP), expressed, purified. The smallest MBP-IntEPEC fusion that efficiently mediated binding HEp-2 cells, monitored by using purified proteins in fluorescence activated cell sorter analysis or fluorescent Covaspheres coated with fusions, contained 150 amino acids Int. Replacement Cys-937 Ser (IntEPEC280CS) destroyed activity IntEPEC280. MBP-IntEPEC280 associated microvilli but failed induce actin accumulation underneath bound particles spreading on plastic surfaces. MBP-IntEPEC280, not MBP, MBP-IntEPEC280CS, MBP-InvYP280, inhibited EPEC entry into cells.
sci-hub.st 参考文章(24)
B B Finlay, I Rosenshine, M S Donnenberg, J B Kaper, Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infection and Immunity. ,vol. 60, pp. 2541- 2543 ,(1992) , 10.1128/IAI.60.6.2541-2543.1992
A E Jerse, J B Kaper, The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infection and Immunity. ,vol. 59, pp. 4302- 4309 ,(1991) , 10.1128/IAI.59.12.4302-4309.1991
M S Donnenberg, J Yu, J B Kaper, A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. Journal of Bacteriology. ,vol. 175, pp. 4670- 4680 ,(1993) , 10.1128/JB.175.15.4670-4680.1993
M S Donnenberg, J B Kaper, Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infection and Immunity. ,vol. 59, pp. 4310- 4317 ,(1991) , 10.1128/IAI.59.12.4310-4317.1991
Ralph R. Isberg, John M. Leong, Multiple β1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells Cell. ,vol. 60, pp. 861- 871 ,(1990) , 10.1016/0092-8674(90)90099-Z
M S Donnenberg, C O Tacket, S P James, G Losonsky, J P Nataro, S S Wasserman, J B Kaper, M M Levine, Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection. Journal of Clinical Investigation. ,vol. 92, pp. 1412- 1417 ,(1993) , 10.1172/JCI116717
Myron M Levine, None, Escherichia coli that Cause Diarrhea: Enterotoxigenic, Enteropathogenic, Enteroinvasive, Enterohemorrhagic, and Enteroadherent The Journal of Infectious Diseases. ,vol. 155, pp. 377- 389 ,(1987) , 10.1093/INFDIS/155.3.377
Martin H. Ulshen, John L. Rollo, Pathogenesis ofEscherichia coliGastroenteritis in Man — Another Mechanism New England Journal of Medicine. ,vol. 302, pp. 99- 101 ,(1980) , 10.1056/NEJM198001103020207
C. L. Francis, A. E. Jerse, J. B. Kaper, S. Falkow, Characterization of Interactions of Enteropathogenic Escherichia coli 0127:H6 with Mammalian Cells In Vitro The Journal of Infectious Diseases. ,vol. 164, pp. 693- 703 ,(1991) , 10.1093/INFDIS/164.4.693
Ralph R. Isberg, Deborah L. Voorhis, Stanley Falkow, Identification of invasin: A protein that allows enteric bacteria to penetrate cultured mammalian cells Cell. ,vol. 50, pp. 769- 778 ,(1987) , 10.1016/0092-8674(87)90335-7