A silver-staining technique for detecting minute quantities of proteins on nitrocellulose paper: retention of antigenicity of stained proteins.

作者: K.C.Leonard Yuen , Terrell K. Johnson , Robin E. Denell , Richard A. Consigli

DOI: 10.1016/0003-2697(82)90534-6

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摘要: Abstract A method using a silver-staining procedure to detect minute quantities of proteins on nitrocellulose paper is described. The technique sensitive enough nanogram resolved sodium dodecyl sulfate-polyacrylamide gels and then transferred by the electrotransfer technique. After staining procedures, are shown retain their antigenic properties.

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