Microfluid system and method for sorting clusters of cells and continuously encapsulating them once they are sorted

摘要: The microfluidic system comprises a substrate in which microchannel network (111, 112, 113, 114) is etched and around protection hood sealed comprising unit for sorting cells. diverting to separate less cohesive cell clusters of size 20-500 mu m, 20-10000 cells such as islets Langerhans, two microchannels arranged parallel outlet the convey many types sorted towards an encapsulation unit. passive fluidic hydrodynamic device. cells, device i.e. hydrodynamically focusing deterministic lateral displacement by arrangement deviation blocks that filtration transversely main microchannel. An (120) automatically encapsulating according their category, formed connecting with continuously forming mechanically resistant selective permeable biocompatible monolayer or multilayer capsule each cluster, sub-units (121, 122, 123, 124) encapsulation, are respectively form predetermined enveloping cluster category. Each sub-unit device, T junction flow-focusing structured micro nozzle capsules, exchanger material between aqueous phase category immiscible phase, oily phase. further gelling capsules. A transfer module present transferring culture medium intended contain contacted minimizing losses coupling connected unit, maintain laminar fluid regime units directly selectively constituted intermediate has dimensions geometry adapted upstream downstream. buffer microreservoir storing open outlet, concentrated equipped valve example air bubble blocking gel so opening closing raises lowers concentration number capsules during formation encapsulation.