Improved method for the recovery of non-segmented, negative-stranded rna viruses from cdna

摘要: Methods for producing infectious, non-segmented, negative-stranded RNA viruses of the Order Mononegavirales are provided that involve coexpression a viral cDNA along with essential proteins, N, P, and L in host cell transiently transfected an expression vector encoding polymerase. In alternate methods, after is one or more vectors polymerase, N protein, P exposed to effective heat shock under conditions sufficient increase recovery recombinant virus. other embodiments, cells transferred rescue begins into co-culture plaque expansion cell, typically monolayer cells, assembled virus recovered from co-culture. Also within invention compositions Mononegavirales, produced using foregoing methods compositions, immunogenic employing viruses. additional employed produce growth- replication-defective non-segmented subviral particles.