Production of Recombinant Proteins in Escherichia coli

作者: Edward R. LaVallie

DOI: 10.1002/0471140864.PS0501S00

关键词:

摘要: E. coli is the expression system of choice and a substantial body literature has accumulated on successful foreign genes in this host. Several problems with protein have been encountered, many ultimately solved. This unit describes methods that developed for production recombinant proteins potential pitfalls may be encountered.

参考文章(51)
A. C. Looman, J. Bodlaender, L. J. Comstock, D. Eaton, P. Jhurani, H. A. de Boer, P. H. van Knippenberg, Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. The EMBO Journal. ,vol. 6, pp. 2489- 2492 ,(1987) , 10.1002/J.1460-2075.1987.TB02530.X
U. Rüther, B. Müller-Hill, Easy identification of cDNA clones. The EMBO Journal. ,vol. 2, pp. 1791- 1794 ,(1983) , 10.1002/J.1460-2075.1983.TB01659.X
B. Nilsson, L. Abrahmsén, M. Uhlén, Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors The EMBO Journal. ,vol. 4, pp. 1075- 1080 ,(1985) , 10.1002/J.1460-2075.1985.TB03741.X
Paul Bornstein, Gary Balian, Cleavage at Asn-Gly bonds with hydroxylamine. Methods in Enzymology. ,vol. 47, pp. 132- 145 ,(1977) , 10.1016/0076-6879(77)47016-2
Harold C. Neu, Leon A. Heppel, The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry. ,vol. 240, pp. 3685- 3692 ,(1965) , 10.1016/S0021-9258(18)97200-5
E.R. LaVallie, A Rehemtulla, L.A. Racie, E.A. DiBlasio, C Ferenz, K.L. Grant, A Light, J.M. McCoy, Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase Journal of Biological Chemistry. ,vol. 268, pp. 23311- 23317 ,(1993) , 10.1016/S0021-9258(19)49464-7
G Fuh, M G Mulkerrin, S Bass, N McFarland, M Brochier, J H Bourell, D R Light, J A Wells, The human growth hormone receptor. Secretion from Escherichia coli and disulfide bonding pattern of the extracellular binding domain. Journal of Biological Chemistry. ,vol. 265, pp. 3111- 3115 ,(1990) , 10.1016/S0021-9258(19)39741-8
G A Bowden, G Georgiou, Folding and aggregation of beta-lactamase in the periplasmic space of Escherichia coli. Journal of Biological Chemistry. ,vol. 265, pp. 16760- 16766 ,(1990) , 10.1016/S0021-9258(17)44825-3
C A Lunn, S Kathju, B J Wallace, S R Kushner, V Pigiet, Amplification and purification of plasmid-encoded thioredoxin from Escherichia coli K12. Journal of Biological Chemistry. ,vol. 259, pp. 10469- 10474 ,(1984) , 10.1016/S0021-9258(18)90987-7
H M Kronenberg, A B Abou-Samra, T J Gardella, S R Nussbaum, D Rubin, H T Keutmann, J T Potts, Expression of human parathyroid hormone-(1-84) in Escherichia coli as a factor X-cleavable fusion protein. Journal of Biological Chemistry. ,vol. 265, pp. 15854- 15859 ,(1990) , 10.1016/S0021-9258(18)55477-6