Conversion of omnipotent translation termination factor eRF1 into ciliate-like UGA-only unipotent eRF1

摘要: In eukaryotic ribosomes, termination of translation is triggered by class 1 polypeptide release factor, eRF1. organisms with a universal code, eRF1 responds to three stop codons, whereas, in ciliates variant codes, only one or two codon(s) remain(s) as signals. By mutagenesis the Y–C–F minidomain N domain, we converted an omnipotent human recognizing all codons into unipotent ‘ciliate-like’ UGA-only The conserved Cys127 located plays critical role codon recognition. response has also been achieved concomitant substitutions four other amino acids at and NIKS minidomains We suggest that for decoding non-linear (non-protein-anticodon) type explores combination positive negative determinants. assume recognition profoundly different prokaryotic RFs.