Complex Formation of DNA with Oppositely Charged Polyelectrolytes of Different Chain Topology: Cylindrical Brushes and Dendrimers

摘要: The complex formation between DNA (pUC19-supercoiled DNA, 2686 base pairs) and some polycations of different chain topologies in aqueous solution was studied by light scattering, gel electrophoresis, AFM. investigated comprised cylindrical brush polymers with quaternized poly(vinylpyridine) polyethylene imine side chains as well a fifth generation dendrimer thus covering broad molar mass regime 3 × 104 g mol-1 < Mw 1 107 very chemical charges/molecule, Z+, 127 Z+ 5500. Irrespective the polycation, complexes formed dilute exhibited similar size terms mean square radius gyration, 〈Rg2〉, i.e., 30 nm Rg 40 (excess DNA) 55 polycation). At large excess either or were shown to coexist uncomplexed molecules component did not vary increasing weight fraction minority component. Only if number ...