THE ROLE OF SURFACE CHEMISTRY IN THE TOXICITY OF MANUFACTURED CERIUM DIOXIDE NANOMATERIALS TO CAENORHABDITIS ELEGANS

摘要: FOR THESIS THE ROLE OF SURFACE CHEMISTRY IN TOXICITY MANUFACTURED CERIUM DIOXIDE NANOMATERIALS TO CAENORHABDITIS ELEGANS Manufactured CeO2 nanomaterials (CeO2-MNMs) are used for a wide variety of applications including diesel fuel additives and chemical/mechanical planarization media. To test the effects CeO2-MNM surface coating charge on to model organism Caenorhabditis elegans, we synthesized 4 nm with cationic (DEAE-), anionic (CM-), neutral (DEX) coatings. In L3 nematodes exposed 24 hours, DEAE-CeO2 induced lethality at lower concentrations than CMor DEX-CeO2. Feeding slightly decreased toxicity, regardless coating. L2 48 hours feeding, caused as compared CMand Sublethal were measured by observing reproduction oxidative/nitrosative protein damage. Low similar reductions DEX-CeO2 that two orders magnitude higher. Using immunochemical slot blots explore stress, no treatments produced significant changes in carbonyl or 3-nitrotyrosine formation; however, statistical power our assay was low. All large but not statistically increases levels. exposure reduction 4-hydroxy-2-nonenol This research suggests coatings render significantly more toxic C. elegans