Effect of the Mi gene on reproduction of Meloidogyne hispanica on tomato genotypes

摘要: {null=The root-knot nematode resistance (Mi) gene was screened in 25 tomato genotypes of Solanum lycopersicum, by amplification REX-1 and Mi23 markers. Ten heterozygous (Mimi), nine homozygous (MiMi) at the Mi locus six lacking for to were identified using marker REX-1. The results obtained with confirmed status genotypes, except genotype Valouro RZ F1 that (Mimi) when markers, respectively. pathogenicity Meloidogyne hispanica on assessed 60 days after inoculation 5000 eggs basis root gall index (GI) reproduction factor (Rf). All susceptible (excellent or good hosts), GI > 4 Rf 2, Rapit considered as resistant/hypersensitive (poor host). In this genotype, induced galls (GI = 4) its roots a small number produced (Pf 3085 ± 485). Significant differences detected between allelic conditions within conditions. increasing alleles (0, 1 2) is associated decreasing Rf, which suggests possible dosage effect gene. variability observed values MiMi may reflect an influence genetic background plants containing are commercially available. However, only can be used control three most common spp. inhibit M. populations, have potential included integrated pest management programme. it advisable evaluate local populations species different environmental factors., en= ( ) lycopersicum , GI>4 Rf>2, (GI=4) (Pf=3085±485). factors. }