Preparation of a low molecular weight polyethylenimine for efficient cell transfection.

作者: Thorsten Bieber , Hans-Peter Elsässer

DOI: 10.2144/01301ST03

关键词:

摘要: Polyethylenimines (PEIs) of a molecular weight between 25 and about 800 kDa have successfully been used for in vitro vivo gene delivery approaches. Recent publications indicated that PEI mol...

参考文章(10)
Hélène Pollard, Jean-Serge Remy, Gildas Loussouarn, Sophie Demolombe, Jean-Paul Behr, Denis Escande, POLYETHYLENIMINE BUT NOT CATIONIC LIPIDS PROMOTES TRANSGENE DELIVERY TO THE NUCLEUS IN MAMMALIAN CELLS Journal of Biological Chemistry. ,vol. 273, pp. 7507- 7511 ,(1998) , 10.1074/JBC.273.13.7507
HUGUES J.-P. RYSER, A Membrane Effect of Basic Polymers dependent on Molecular Size Nature. ,vol. 215, pp. 934- 936 ,(1967) , 10.1038/215934A0
A Baker, M Saltik, H Lehrmann, I Killisch, V Mautner, G Lamm, G Christofori, M Cotten, Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Therapy. ,vol. 4, pp. 773- 782 ,(1997) , 10.1038/SJ.GT.3300471
O. Boussif, F. Lezoualc'h, M. A. Zanta, M. D. Mergny, D. Scherman, B. Demeneix, J. P. Behr, A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 7297- 7301 ,(1995) , 10.1073/PNAS.92.16.7297
Bassima Abdallah, Ahmed Hassan, Corinne Benoist, Daniel Goula, Jean Paul Behr, Barbara A. Demeneix, A Powerful Nonviral Vector for In Vivo Gene Transfer into the Adult Mammalian Brain: Polyethylenimine Human Gene Therapy. ,vol. 7, pp. 1947- 1954 ,(1996) , 10.1089/HUM.1996.7.16-1947
Ernst Wagner, Effects of membrane-active agents in gene delivery Journal of Controlled Release. ,vol. 53, pp. 155- 158 ,(1998) , 10.1016/S0168-3659(97)00249-6
W. T. Godbey, K. K. Wu, A. G. Mikos, Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 5177- 5181 ,(1999) , 10.1073/PNAS.96.9.5177
Richard N. Day, Margaret Kawecki, Diana Berry, Dual-function reporter protein for analysis of gene expression in living cells BioTechniques. ,vol. 25, pp. 848- 856 ,(1998) , 10.2144/98255BT02