Recruitment and Endo-Lysosomal Activation of TLR9 in Dendritic Cells Infected with Trypanosoma cruzi

摘要: TLR9 is critical in parasite recognition and host resistance to experimental infection with Trypanosoma cruzi. However, no information available regarding nucleotide sequences cellular events involved on T. cruzi by TLR9. In silico wide analysis associated vitro screening of synthetic oligonucleotides demonstrates that the retrotransposon VIPER elements mucin-like glycoprotein (TcMUC) genes genome are highly enriched for CpG motifs immunostimulatory mouse human TLR9, respectively. Importantly, triggers high levels luciferase activity under NF-κB-dependent transcription HEK cells cotransfected but not control (cotransfected MD2/TLR4) cells. Further, we observed translocation lysosomes during invasion/uptake parasites dendritic Consistently, potent proinflammatory was when unmethylated genomic DNA delivered endo-lysosomal compartment expressing Thus, together our results indicate found likely be main targets probably become destroyed lysosome-fused vacuoles phagocytes.