THE FRACTIONATION OF PROTEIN MIXTURES BY LINEAR POLYMERS OF HIGH MOLECULAR WEIGHT.
作者： A. Polson , G.M. Potgieter , J.F. Largier , G.E.F. Mears , F.J. Joubert
DOI: 10.1016/0304-4165(64)90438-6
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摘要: Abstract The protein fractionating properties of the high molecular weight linear polymers, polyethylene glycol, dextran, nonylphenol-ethoxylate, polyvinyl alcohol and pyrrolidone have been investigated. All were precipitants, but varied in selectivity. Polyethylene glycol (mol. wt. 6000) appears to be most suitable precipitants this group because its solutions are less viscous cause virtually no denaturation at room temperature. efficiency fractionation increases with weight, therefore chain length, polymer. Rapid simple methods for preparation γ-globulin fibrinogen aid described. products homogeneous on electrophoresis centrifugation retained satisfactory biological properties.
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sci-hub.se 参考文章(7)
ALFRED POLSON, A New Method for Measuring Diffusion Constants of Biologically Active Substances Nature. ,vol. 154, pp. 823- 823 ,(1944) , 10.1038/154823A0
E. L. Durrum, A Microelectrophoretic and Microionophoretic Technique1 Journal of the American Chemical Society. ,vol. 72, pp. 2943- 2948 ,(1950) , 10.1021/JA01163A037
A. Polson, An electrophoresis cell for analysing four samples simultaneously. Biochimica et Biophysica Acta. ,vol. 13, pp. 451- 452 ,(1954) , 10.1016/0006-3002(54)90355-8
John F. Largier, A purification and investigation of tetanus antitoxin Archives of Biochemistry and Biophysics. ,vol. 77, pp. 350- 367 ,(1958) , 10.1016/0003-9861(58)90082-1
Alfred Polson, Multi-membrane electrodecantation and its application to isolation and purification of proteins and viruses. Biochimica et Biophysica Acta. ,vol. 11, pp. 315- 325 ,(1953) , 10.1016/0006-3002(53)90050-X
E. J. Cohn, F. R. N. Gurd, D. M. Surgenor, B. A. Barnes, R. K. Brown, G. Derouaux, J. M. Gillespie, F. W. Kahnt, W. F. Lever, C. H. Liu, D. Mittelman, R. F. Mouton, K. Schmid, E. Uroma, A System for the Separation of the Components of Human Blood: Quantitative Procedures for the Separation of the Protein Components of Human Plasma1a,b,c Journal of the American Chemical Society. ,vol. 72, pp. 465- 474 ,(1950) , 10.1021/JA01157A122
Samuel Losner, Bruno W. Volk, Rudolph E. Fremont, Alice Einhorn, Studies on the Fibrinogen Polymerization Test American Journal of Clinical Pathology. ,vol. 27, pp. 609- 618 ,(1957) , 10.1093/AJCP/27.6.609