Transcriptome and proteome analysis of xylose-metabolising Saccharomyces cerevisiae

摘要: Increasing concern about global climate warming has accelerated research into renewable energy sources that could replace fossil petroleum-based fuels and materials. Bioethanol production from cellulosic biomass by fermentation with baker’s yeast Saccharomyces cerevisiae is one of the most studied areas in this field. The focus been on metabolic engineering S. for utilisation pentose sugars, particular D-xylose abundant hemicellulose fraction biomass. Introduction a heterologous xyloseutilisation pathway enables xylose fermentation, but ethanol yield productivity do not reach theoretical level. In present study, transcription, proteome flux analyses recombinant xylose-utilising expressing genes encoding reductase (XR) xylitol dehydrogenase (XDH) Pichia stipitis endogenous xylulokinase were carried out to characterise cellular responses metabolism xylose. aim these studies was find novel ways engineer cells improved fermentation. grown glucose both batch chemostat cultures. A particularly interesting observation several proteins had post-translationally modified forms different abundance glucose. Hexokinase 2, glucokinase enolase isoenzymes 1 2 phosphorylated differently two carbon studied. This suggests phosphorylation glycolytic enzymes may be yet poorly understood means modulate their activity or function. results also showed affected gene expression pathways leading acetyl-CoA synthesis altered fluxes pathways. Additionally,