Fast Photochemical Oxidation of Proteins for Epitope Mapping
作者： Lisa M. Jones , Justin B. Sperry , James A. Carroll , Michael L. Gross
DOI: 10.1021/AC2007366
关键词:
摘要: The growing use of monoclonal antibodies as therapeutics underscores the importance epitope mapping an essential step in characterizing antibody-antigen complexes. protein footprinting coupled with mass spectrometry, which is emerging a tool structural biology, offers opportunities to map antibody-binding regions antigens. We report here via fast photochemical oxidation proteins (FPOP) OH radicals characterize serine-protease thrombin. data correlate well previously published results that determined This study marks first time oxidative labeling has been used for mapping.
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