Metabolite and gene expression profiles suggest a putative mechanism through which high dietary carbohydrates reduce the content of hepatic betaine in Megalobrama amblycephala.

摘要: High-carbohydrate diets (HCD) are favoured by the aquaculture industry for economic reasons, but they can produce negative impacts on growth and induce hepatic steatosis. We hypothesised that mechanism behind this is reduction of betaine content. further explored supplementing (1%) to diet a farmed fish Megalobrama amblycephala. Four groups were designed: control (CD, 27.11% carbohydrates), high-carbohydrate (HCD, 36.75% long-term (LBD, 35.64% carbohydrates) short-term (SBD; 12 weeks HCD + 4 weeks LBD). analysed performance, body composition, liver condition, expression genes profiles metabolites associated with metabolism. HCD resulted in poorer health (compared CD), whereas LBD improved these parameters HCD). induced glucose, serine cystathionine metabolisms, (non-significantly, p = .20) betaine-catabolizing enzyme betaine-homocysteine-methyltransferase; decreased content betaine, methionine, S-adenosylhomocysteine carnitine. Betaine supplementation (LBD) reversed patterns, elevated betaine-homocysteine-methyltransferase, S-adenosylmethionine (all p ≤ .05). hypothesise reduced enhancing activity metabolic pathways from glucose homocysteine, reflected increased glycolysis, metabolism, metabolism homocysteine remethylation. Long-term dietary HCD, inculding parameters, However, may have caused temporary disruption homeostasis.