Chitosan–cellulose composite membrane for affinity purification of biopolymers and immunoadsorption

摘要: Abstract A composite chitosan–cellulose membrane was prepared by coating chitosan on filter paper. Physical properties of the and its application in affinity immunoadsorption (IgG to immobilized Protein A) were examined. The porogen (polyethylene glycerol, PEG) contents preparation solution have a significant effect porosity flow rate water through membrane. evaporation time (ET) after also determined. with relatively large flux (3.2 ml/min/cm 2 at 12.5 psi) approximately 1 μm pore size using 0.5% containing 15.0% PEG evaporating for 3 h room temperature coating. obtained provided tensile strength larger than plain cellulose support, both wet dry states. hydroxyl groups modified an 11-atom spacer arm link as ligand. For comparison, paper linked same method. amount 1.36 6.29 mg/ml membrane, respectively. high dynamic binding capacity human IgG 14.9 mg/ml which is four-fold higher that