Hybrid cell extinction and re‐expression of Oct‐3 function correlates with differentiation potential.
作者： T. Shimazaki , H. Okazawa , H. Fujii , M. Ikeda , K. Tamai
DOI: 10.1002/J.1460-2075.1993.TB06138.X
关键词:
摘要: The Oct-3 gene is expressed in highly undifferentiated cells and implicated mammalian early embryogenesis. We have generated a series of hybrid between pluripotent embryonal carcinoma (Oct-3+) fibroblasts (Oct-3-), studied the regulation function Oct-3. Upon fusion, differentiated to nestin+/Brn-2+ resembling neuroepithelial stem cells. Expression was extinguished at transcriptional level all examined. modulating activity required for Oct-3-mediated enhancer activation also extinguished. When transactivating introduced into cells, they transformed morphologically distinct nestin-/Brn-2- ('revertants'). 'revertant' subsequently lost expression, back close correlation phenotypic changes gain/loss indicates that can induce dedifferentiation neural
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