Functional neuroimaging in rodents using cerebral blood flow SPECT

摘要: The brain-wide activation patterns that underlie, generate and change the behavioral outputs in rodents, main animal models biomedical research, are difficult to assess vivo. standard tool for whole-brain imaging of spatiotemporal rodents is BOLD fMRI, but technique requires animals be immobilized inside scanners. One few methods can provide vivo images neural activity from unrestrained outside scanner environments single-photon emission computed tomography (SPECT) cerebral blood flow (CBF). During ongoing behavior intravenously injected with 99mTc-HMPAO (99m-technetium hexamethylene propyleneamine oxime), a lipophilic tracer that, after accumulation brain flow-dependent manner, rapidly converted hydrophilic compound remains trapped shows no redistribution. 99mTc distribution, reflecting average during time injection, read out anesthetized injection. Similar rationale 18F-2-fluoro-2-deoxy-glucose (18F-FDG) positron (PET) SPECT provides static spatial awake behaving resolution higher stimulation times substantially shorter. In this review we present an overview about underlying rationales principles functional CBF give short summary experimental procedures discuss advantages, drawbacks perspectives within framework rodents.