Combined Nested RT-PCR Assay for Prostate-specific Antigen and Prostate-specific Membrane Antigen in Prostate Cancer Patients: Correlation with Pathological Stage

摘要: Nested reverse transcription (RT)-PCR for prostate-specific antigen (PSA) and membrane (PSM) can detect circulating prostatic cells in patients with prostate cancer. We evaluated the role of a combined screening approach PSA PSM cancer staging. examined peripheral blood samples from 136 adenocarcinoma (PCA), 15 benign hyperplasia, normal male subjects, 5 female subjects. The controls (benign hyperplasias, males, females) were negative both PSM. In metastatic PCA (n = 11), 100% positive by PSA/PSM (64% 91% PSM). biochemical failure 18), 39% PSM, compared to only 6% PSA. clinically localized 107), 48% (43% 14% PSA). These results show that is more sensitive marker than detecting (P < 0.0001). correlated preoperative RT-PCR final pathological stages 67 prostatectomy patients. positivity was 81.5% non-organ-confined disease versus 37.5% organ-confined 0.001). had an odds ratio 7.3 (95% confidence interval, 2.3-23.4; P 0.001) predicting tumor extracapsular extension. better predictor extension initial serum PSA, clinical stage, biopsy Gleason score. Our data nested may provide staging information unavailable current modalities. ultimate impact this technique management will require continued investigation.