Polymorphonuclear leukocyte membrane fluidity and cytosolic Ca(2+) content in young adults with acute myocardial infarction. Evaluation at the initial stage and after 12 months.
作者： G. Caimi , B. Canino , E. Incalcaterra , R. Lo Presti , M. Montana
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摘要: Our aim was to examine two aspects of polymorphonuclear leukocyte (PMN) rheology (membrane fluidity and cytosolic Ca 2 + content), at baseline after in vitro activation, a group young adults with acute myocardial infarction (AMI) the initial stage 12 months. We enrolled 21 AMI subjects aged ≤ 45 years (mean age 41.1 ′ 3.5 years) evaluated PMN membrane fluidity, labelling intact cells fluorescent probe 1,4-(trimethylamino)-phenyl-4-phenylhexatriene content marking Fura 2-AM, activation 4-phorbol 12-myristate 13-acetate (PMA) N-formyl-methionyl-leucyl-phenylalanine (fMLP). During did not distinguish patients from control subjects; months, when compared stage, significantly increased. In PMA fMLP caused no variation parameters subjects, while decreased increased; same behaviour pattern observed The constant functional alteration highlights role activated leukocytes as component inflammatory reaction that follows ischemia.
eurekamag.info 参考文章(25)
G. Caimi, B. Canino, R. Lo Presti, M. Montana, E. Hoffmann, F. Dispensa, A. Catania, Haemorheological pattern in young adults with acute myocardial infarction. Clinical Hemorheology and Microcirculation. ,vol. 29, pp. 11- 18 ,(2003)
R. C. Schlant, Sandra Forman, J. Stamler, P. L. Canner, The Natural History of Coronary Heart Disease: Prognostic Factors After Recovery from Myocardial Infarction in 2789 Men Springer, Berlin, Heidelberg. pp. 74- 93 ,(1985) , 10.1007/978-3-642-70296-9_7
R.L. Prentice, T.P. Szatrowski, H. Kato, M.W. Mason, Leukocyte counts and cerebrovascular disease. Journal of Chronic Diseases. ,vol. 35, pp. 703- 714 ,(1982) , 10.1016/0021-9681(82)90094-7
M.W Roe, J.J Lemasters, B Herman, Assessment of Fura-2 for measurements of cytosolic free calcium Cell Calcium. ,vol. 11, pp. 63- 73 ,(1990) , 10.1016/0143-4160(90)90060-8
Christopher P Cannon, Carolyn H McCabe, Robert G Wilcox, Jane H Bentley, Eugene Braunwald, Association of white blood cell count with increased mortality in acute myocardial infarction and unstable angina pectoris American Journal of Cardiology. ,vol. 87, pp. 636- 639 ,(2001) , 10.1016/S0002-9149(00)01444-2
J W Yarnell, P M Sweetnam, P C Elwood, R Eastham, R A Gilmour, J R O'Brien, M D Etherington, Haemostatic factors and ischaemic heart disease. The Caerphilly study. Heart. ,vol. 53, pp. 483- 487 ,(1985) , 10.1136/HRT.53.5.483
Kozo Yasui, Midori Masuda, Takafumi Matsuoka, Munehiro Yamazaki, Atsushi Komiyama, Taro Akabane, Masafumi Hasui, Yohnosuke Kobayashi, Kenjiro Murata, Abnormal membrane fluidity as a cause of impaired functional dynamics of chemoattractant receptors on neonatal polymorphonuclear leukocytes : lack of modulation of the receptors by a membrane fluidizer Pediatric Research. ,vol. 24, pp. 442- 446 ,(1988) , 10.1203/00006450-198810000-00005
Richard H. Grimm, Prognostic Importance of the White Blood Cell Count for Coronary, Cancer, and All-Cause Mortality JAMA: The Journal of the American Medical Association. ,vol. 254, pp. 1932- 1937 ,(1985) , 10.1001/JAMA.1985.03360140090031
M. Shinitzky, Y. Barenholz, Fluidity parameters of lipid regions determined by fluorescence polarization Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. ,vol. 515, pp. 367- 394 ,(1978) , 10.1016/0304-4157(78)90010-2
JL Mehta, DY Li, Inflammation in ischemic heart disease: Response to tissue injury or a pathogenetic villain? Cardiovascular Research. ,vol. 43, pp. 291- 299 ,(1999) , 10.1016/S0008-6363(99)00132-7