Detection of phosphate esters on paper chromatograms.

摘要: PHOSPHATE esters separated on paper chromatograms are usually detected by degrading them the with acid to give ortho-phosphoric and detecting this reaction molybdate a reducing reagent. The procedure of Hanes Isherwood1 is based upon widely used. This technique suffers from two disadvantages. In first place, prolonged initial ‘digestion’ necessary break down more resistant frequently leaves in very fragile state; secondly, further analyses cannot be carried out spot after treatment. order avoid these disadvantages following was developed. It depends fixation ferric ions free ion salicylsulphonic acid. If not strongly buffered it sprayed 0.1 per cent FeCl3.6H2O 80 alcohol, dried air at room temperature then 1 alcohol. Upon drying, phosphates appear as white spots pale mauve background, ortho-phosphate having band deeper surrounding it. colour formation occurs only when pH residual moisture about 1.5–2.5. can checked conveniently thymol blue, since most satisfactorily just turns indicator red. Further indications unsatisfactory that below range no formed, whereas above orange-yellow definition poor.