Interbacterial Adhesion Networks within Early Oral Biofilms of Single Human Hosts.

摘要: Specific interbacterial adhesion, termed coaggregation, is well established for three early colonizers of the plaque biofilm: streptococci, actinomyces, and veillonellae. However, little known about interactions other extent within bacterial community from a single host. To address these gaps, subject-specific culture collections two individuals were using an intraoral biofilm retrieval device. Molecular taxonomy (Human Oral Microbe Identification Microarray [HOMIM]) analysis samples confirmed integrity completeness collections. HOMIM verified isolation Streptococcus gordonii S. anginosus only one subject, as previously uncultivated streptococcal phylotype subject. Strains representative clonal diversity each collection further characterized. Greater than 70% strains subject coaggregated with at least coisolate. One-third carry coaggregation mediator: receptor polysaccharide (RPS). Almost all nonstreptococcal isolates coisolates. Importantly, certain Rothia demonstrated more coaggregations their coisolated bacteria did any or Actinomyces strain, Haemophilus participated in twice many. Confocal microscopy undisturbed biofilms showed that occur small multispecies microcolonies. confluent high-biomass regions, occurred islands whereas was distributed throughout. Together, data demonstrate networks individual's oral microflora are extensive can be important initiators cell-cell biofilm.IMPORTANCE Extensive involvement specific adhesion dental formation has been postulated based on vitro between not specific. In present study, obtained volunteers, assayed. Coaggregations, several which involved coaggregation-mediating cell surface molecule well-studied widespread. Unexpectedly, little-studied organisms greatest numbers members; different distributions biofilm. The show encompass most individual, they indicate prominent participation such formation.