Paper-Based Bipolar Electrode Electrochemiluminescence Switch for Label-Free and Sensitive Genetic Detection of Pathogenic Bacteria.
作者： Hongxing Liu , Xiaoming Zhou , Weipeng Liu , Xiaoke Yang , Da Xing
DOI: 10.1021/ACS.ANALCHEM.6B02772
关键词:
摘要: Genetic analysis is of great importance for the detection pathogenic bacteria. Bacterial identification must become simpler, less expensive, and more rapid than traditional methods. In this study, a low-cost, label-free, wireless paper-based bipolar electrode electrochemiluminescence (pBPE-ECL) system was constructed sensitive genetic Wax-screen printing used to form hydrophilic channels on filter paper, carbon ink-based driving electrodes were screen-printed into channels. The “light-switch” molecule [Ru(phen)2dppz]2+ (phen = 1,10-phenanthroline; dppz dipyridophenazine) intercalate base pairs double-stranded DNA PCR amplification products, complexs then applied perform ECL assays; quenched in aqueous solution, but displays intense when intercalated double-str...
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