Optimization of a nanostructured surface for the development of electrochemical immunosensors

摘要: Abstract It is known that in the development of electrochemical immunosensors, electrode used both for immobilization antibodies and carrying out redox reaction. Therefore, it important to have an electrical conductive surface with possibility retaining antibodies. To achieve a better sensitivity greater work concentration range, can be modified different appropriate materials such as multi-walled carbon nanotubes (MWCNT) gold nanoparticles (AuNPs) among others. In this work, design platform immunosensor was studied. Thus, optimization screen printed electrodes dispersion MWCNT polyethylenimine (MWCNT/PEI) AuNPs performed, through multivariate experimental design. A factorial 32 levels used, where variables were volumes solution MWCNT/PEI given concentrations, respectively. The optimized responses cathodic peak current reduction K3[Fe(CN)6] charge transfer resistance K4[Fe(CN)6]/K3[Fe(CN)6] couple, using cyclic voltammetry impedance spectroscopy. fitted lineal model, while by quadratic model. desirability function determine lower higher K3[Fe(CN)6]. nanostructured promises suitable structure new immunosensor.