Hyposialylation of differentiation-inducer-resistant HL-60 cells.

摘要: The total sialic acid concent of retinoic (RA)-resistant or 6- thioguanine (6TG)-resistant HL-60 cells was more than tenfold lower and dimethylsulfoxide (DMSO)-resistant approximately twofold that parental, wild-type (wt) cells. Neuraminidase-inaccessible, ie residual cell-associated after neuraminidase treatment, four- to twelvefold in the three differentiation-inducer-resistant sublines parent line. Neuraminidase treatment 125I-labeled surface membrane glycoproteins (SMGs) from wt converted two-dimensional gel electrophoretic pattern one having features common with RA- 6TG-resistant However, did not alter sensitivity differentiation induction by RA, hypoxanthine (purine base), DMSO. These results indicate differences peripheral, neuraminidase-accessible acids are important determinants mobility SMGs line but likely related differentiation-resistance mechanism. Further studies required determine if hyposialylation cryptic, neuraminidase-inaccessible sites has functional significance.