Role of reactive oxygen species and cardiolipin in the release of cytochrome c from mitochondria
作者： Giuseppe Petrosillo , Francesca M. Ruggiero , Giuseppe Paradies
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摘要: Several lines of evidence indicate that mitochondria-mediated reactive oxygen species (ROS) generation is a major source oxidative stress in the cell. Release cytochrome c from mitochondria central event apoptosis induction and appears to be mediated by ROS. Dissociation IMM, where it bound cardiolipin, represents necessary first step for release. In present study, role ROS cardiolipin release rat liver was investigated. were produced oxidizing succinate nonphosphorylating state. Cytochrome quantitated new, very sensitive rapid reverse-phase HPLC method. We found succinate-supported production resulted parallel loss content. These effects directly significantly correlated also abolished ADP, which prevents succinate-mediated production. The ROS-induced independent MPT involve VDAC. It suggested mitochondrial-induced promotes two-steps process, consisting dissociation this protein followed permeabilization outer membrane, probably interaction with data may help clarify molecular mechanism underlying cytosol
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