Mutant g-protein coupled receptors and methods for selecting them
作者： Maria Josefa Serrano-Vega , Richard Henderson , Yoko Shibata , Malcolm Peter Weir , Anthony Johannes Warne
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摘要: A method for selecting a G-protein coupled receptor (GPCR) with increased stability, the comprising (a) providing one or more mutants of parent GPCR, (b) ligand, ligand being which binds to GPCR when is residing in particular conformation, (c) determining whether each mutant has stability respect binding selected compared that and (d) those have an ligand. Mutants β-adrenergic receptor, adenosine neurotensin are also disclosed.
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