Characterization of putative G1/S transcription complex factors Swi6p, Swi4p and Mbp1p in the fungal pathogen Candida albicans

摘要: The G1/S transition governs cell proliferation in many systems, and involves essential transcription complexes such as SBF MBF Saccharomyces cerevisiae, or Schizosaccharomyces pombe. are composed of the regulatory subunit Swi6p DNA-binding elements Swi4p (SBF) Mbp1p (MBF). fungal pathogen Candida albicans contains orthologues SWI6, SWI4 MBP1. Previous genetic, DNA expression bioinformatic data suggest that may be core components a single MBF-like complex. However, direct evidence is lacking, role unclear. In order to determine composition mechanisms action putative complex C. albicans, identify other factors important for control, we determined physical interactions between Swi6p, using co-immunoprecipitation, systematically affinity-purified each protein identified interacting through mass spectrometry, investigated targets genome wide location analysis (ChIP-chip). We show physically interacts with Mbp1p, bind Mbp1p. Affinity purifications did not additional proteins, suggesting factors. Finally, ChIP-chip including G1 cyclins, wall-associated factors, regulators hyphal growth, consistent swi4/ phenotype. Unexpectedly, few target promoters contained conserved binding site (ACGCGT). Rather, 8.6% canonical SBF-binding (CNCGAAA) motif, 43.0% related CACAAAA. Although require confirmation by qPCR, results involved regulating progression development, but exclusively function MBF-binding element. summary, our provide new insights on regulation which have implications controlling development pathogen.