The F0 Complex of the Escherichia Coli ATP Synthase

摘要: The antigenic determinants of mAbs against subunit c the Escherichia coli ATP synthase were mapped by ELISA using overlapping synthetic heptapeptides. All epitopes recognized are located in hydrophilic loop region and as follows: 31-LGGKFLE-37, 35-FLEGAAR-41, 36-LEGAAR-41 36-LEGAARQ-42. Binding studies with membrane vesicles different orientation revealed that all bind to everted independent presence or absence F1 part. Although particularly highly conserved residues A40, R41, Q42 P43 known interact subunits γ e part, mAb molecules have no effect on function F0. Furthermore, it could be demonstrated part molecule(s) bound simultaneously F0 complex suggesting not involved interaction. From results obtained, can concluded this interaction is fixed, which means do switch between during catalysis furthermore, a complete rotation oligomer modified mAb(s) along stator F1F0 complex, proposed composed at least b δ, seems unlikely.