Chemoattractant-induced cytoplasmic pH changes and cytoskeletal reorganization in human neutrophils. Relationship to the stimulated calcium transients and oxidative burst.

摘要: The relationships between the chemotactic factor-stimulated mobilization of calcium, activation NADPH-oxidase, changes in cytosolic pH, and level polymerized actin human neutrophils have been examined. approach taken was to use intracellular calcium chelators, pharmacologic modulators (both positive negative) NADPH-oxidase measure aforementioned responses under conditions where transients were abrogated and/or generation superoxide anions either inhibited or augmented. decrease pH induced by chemoattractants chelator BAPTA diglyceride kinase inhibitor 6-[2-(4-[(4-fluorophenyl)phenylmethylene]-1-piperidinylethyl ]-7-methyl-5H-thiazolo[3,2-alpha]pyriimidin-5-one (R59022) (this latter compound enhanced oxidative response cells). Furthermore, a specific (diphenyleneiodonium) had no significant effect on acidification FMLP leukotriene B4. These results indicate that initiation factors is calcium-dependent event not directly linked NADPH-oxidase. In contrast, stimulated polymerization insensitive BAPTA, R59022, diphenyleneiodonium. Thus, neither nor burst play signaling role elicited chemoattractants. data additional investigations are needed uncover biochemical basis signals initiated lead acidification.