Thermoresponsive artificial extracellular matrix for tissue engineering: hyaluronic acid bioconjugated with poly(N-isopropylacrylamide) grafts.

摘要: Thermoresponsive hyaluronans (HAs) were prepared by graft polymerization of N-isopropylacrylamide (NIPAM) on HA (number-averaged molecular weight, Mn, ca. 1.5 × 105 and 5.0 105) using dithiocarbamate which is a kind iniferter (initiator, transfer agent terminator). The degree dithiocarbamylation (DD) as an ranged from 0.4 to 11.4% per disaccharide unit HA. estimated Mn the grafted polyNIPAM (PNIPAM) approximately 103 8.4 104. PNIPAM-grafted HAs (PNIPAM-HAs) water-soluble at room temperature, while they precipitated temperatures above 34 °C in water. temperature onset precipitation (lower critical solution temperature:  LCST) was independent parameters architecture such HA, grafting PNIPAM, PNIPAM. Equilibrium transmittance aqueous LCST decreased with increase both At physiological PNI...