Expression of prolactin receptor and response to prolactin stimulation of human NK cell lines

摘要: We have previously shown a critical role of prolactin (PRL) during maturation and anti-tumor effects murine natural killer (NK) cells in vitro vivo. extended that study by exploring the ability human NK cell lines (NK-92 YT cell) to express PRL receptor (PRL-R) respond stimulation vitro. Both constitutively expressed PRL-R on membrane mRNA transcripts, NK-92 contained higher level than cells, which correlated enhanced capacity proliferate lyse target response presence trace amount IL-2 or IL-15 Two differences between functioning were for first time observed. synergized with improve proliferation dose-dependent manner without double peak manifesting like IL-2. Although cytotoxicity activated it exerted function through up-regulating gene expression perforin influence FasL IL-2-stimulated while IL-15-stimulated did both but not IFN-gamma. increased expressions IL-2Ralpha indicating up-regulated improving positive feedback IL-2R. The similar results also observed network IL-15R. These data indicate potential modulation.