The Role of T Cells in Anti-Herpes Simplex Virus Immunity I. Induction of Antigen-Specific Cytotoxic T Lymphocytes

摘要: Mice infected with herpes simplex virus develop little or no cytotoxic T lymphocyte (CTL) response. However, in lymph nodes (LN9s) draining a local site HSV, antigen-specific CTL precursors are sensitized, which upon transfer to vitro culture conditions within 72 hr into effective CTL. The vivo blockade of differentiation can be overcome by cyclophosphamide, suggesting that cyclophosphamide-sensitive mechanism blocks the generation HSV-immune cytolytic activity is H-2 restricted and antigen specific. Thus sensitized toward HSV type 1 discriminate between syngeneic targets either immunologic variant 2 (and vice versa ). H-2-matched target cells exposed for 30 min infectious lysed 60 contact Since replication believed require more than 4 5 hr, data suggest expression HSV-dependent “early proteins” takes place 90 cell membrane-integrated virion represents