The chromatography of the flavonoid pigments

摘要: Paper partition chromatography was first applied to the separation of flavonoid pigments by BATE-SMIT+~~ in 1948. Since then, it has been used increasingly study these compounds, which are ideally suited this particular technique. The flavonoids have just right range solubility characteristics for ease and most them possess characteristic colours on paper visible or ultraviolet light. thus provided satisfactory means surveying plant material presence water-soluble pigment&4. It particularly valuable separating components mixtures flavonoids, occur some plants (e.g.51s). Large scale separations carried out adsorption cellulose magnesol columns7r8. But is characterisation elucidation structure unknown a micro-scale that so successful 5~6*** lo. identification therefore major topic review. A large number RF values given new ones determined laboratory included. term here describe group phenolic sometimes known as anthoxanthins. These widely occurring flavones flavonols less frequently distributed flavanones, isoflavones, chalcones aurones. Flavans (monomeric leucoanthocyanins catechins) other related substances may also be considered fall into category. anthocyanins, associated with structurally anthoxanthins, already discussedll. In order able relate value (section 4), brief consideration chemical must made. formulae commonly compounds shown Fig. I. Examples included all main classes generally differ from each oxidation level central pyran ring. Within classes, structural variation mainly confined differences position hydroxyl, methyl glycosyl substituents. There considerable rare additional substituents (e.g. methylene-dioxy groups, isoprene side chains, etc.). Their structures recent review naturally GEISSMAN AND HINREINER~~.