Engineering of Corynebacterium glutamicum to utilize methyl acetate, a potential feedstock derived by carbonylation of methanol with CO.
作者： Seungjung Choo , Youngsoon Um , Sung Ok Han , Han Min Woo
DOI: 10.1016/J.JBIOTEC.2016.03.011
关键词:
摘要: The possibilities to utilize one-carbon substrates (C1) like CO, methane and methanol have been explored as a cheap alternative feedstock in the biotechnology. For first time, methyl acetate (MeOAc), which can be formed from carbonylation of with was demonstrated an carbon source for cell growth Corynebacterium glutamicum model microbial factory. To do so, carboxyl esterase activity necessary hydrolyze MeOAc acetate. Although wild-type has unknown MeOAc, not high enough grow 270mM sole source, reaching OD600 5.28±0.2 32h. Based on literatures studied esterase, we chose three esterases (MekB Pseudomonas veronii MEK700, AcmB Gordonia sp. Strain TY-5, Est Pyrobaculum calidifontis VA1) cloned into wild-type. As result, recombinant C. expressing highly active MekB (28.6±0.77U/mg protein) showed complete degradation utilization acetate, resulting 16.5±0.02at 24h. In addition, strain exhibited rapid 2h under anaerobic condition. Therefore, used another C1-derived
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