DNA binding hydroxyl radical probes

摘要: Abstract The hydroxyl radical is the primary mediator of DNA damage by indirect effect ionizing radiation. It a powerful oxidizing agent produced radiolysis water and responsible for significant fraction associated with There therefore an interest in development sensitive assays its detection. hydroxylation aromatic groups to produce fluorescent products has been used this purpose. We have examined four different chromophores, which when hydroxylated. Of these, coumarin system suffers from fewest disadvantages. behavior linked cationic peptide ligand designed bind strongly DNA.