miR-137 acts as a tumor suppressor in astrocytoma by targeting RASGRF1

摘要: Astrocytoma is one of the most common primary central nervous system tumors and has both high mortality a poor 5-year survival rate. MicroRNAs (miRNAs) play important roles in carcinogenesis by acting on multiple signaling pathways. Although we have demonstrated that miR-137 downregulated astrocytoma tissues, role still remains unknown. In present study, aimed to investigate function its possible target genes astrocytoma. was significantly expression level inversely correlated with clinical stage. Restoring able dramatically inhibit cell proliferation, migration, invasion enhance apoptosis vitro, whereas silencing inhibited these processes. By overexpressing or inhibiting cancer cells, experimentally confirmed directly recognized 3'-UTR (3'-untranslated region) RASGRF1 (Ras protein-specific guanine nucleotide-releasing factor 1) transcript regulated expression. Furthermore, an inverse correlation observed between levels protein levels, but not mRNA samples. The resulted similar effects restoration cells. Finally, overexpression rescued inhibitory miR-137. Taken together, our results indicate acts as tumor suppressor targeting RASGRF1. These findings suggest may serve novel therapeutic treatment.