Proteomic investigation of the effects of weight loss in the gastrocnemius muscle of wild and NZW rabbits via 2D-electrophoresis and MALDI-TOF MS.

摘要: Summary The study of changes within the key agents regulating metabolism during genetic upgrading because selection can contribute to an improved understanding genomic and physiological relationships. This may lead increased efficiencies in animal production. These changes, regarding energy protein metabolic saving mechanisms, be highlighted food restriction periods. In this study, a 20% weight reduction was induced two rabbit breeds: New Zealand white, selected meat producer (Oryctolagus cuniculus cuniculus), Iberian wild algirus), with aim determining differential expression gastrocnemius muscle control (ad libitum) restricted diet experimental groups, using techniques two-dimensional gel electrophoresis peptide mass fingerprinting. Results show that L-lactate dehydrogenase, adenylate kinase, b enolase enolase, fructose bisphosphate aldolase A glyceraldehyde 3-phosphate which are enzymes involved metabolism, differentially expressed groups. available further tested as relevant biomarkers loss putative objects manipulation tool towards increasing tolerance loss. Similar reasoning could applied 2D spots corresponding important structural proteins tropomyosin chain troponin I. Finally, spot identified mitochondrial import stimulation factor seems special interest marker undernutrition, it object studies aiming better understand its role.