Concentration-dependent antagonistic persuasion of SDS and naphthalene derivatives on the fibrillation of stem bromelain
作者： Atiyatul Qadeer , Ejaz Ahmad , Masihuz Zaman , Mohd Wasif Khan , Javed Masood Khan
DOI: 10.1016/J.ABB.2013.10.015
关键词:
摘要: Abstract Sodium dodecyl sulfate, a biological membrane mimetic, can be used to study the conversion of globular proteins into amyloid fibrils in vitro . Using multiple approaches, effect SDS was examined on stem bromelain (SB), widely recognized therapeutic protein. SB is known exist as partially folded intermediate at pH 2.0, situation also encountered gastrointestinal tract (its site absorption). In presence sub-micellar concentration (500–1000 μM), this found exhibit great propensity form large-sized β-sheeted aggregates with fibrillar morphology, hall marks structure. We observed inhibition fibrillation by two naphthalene-based compounds, ANS and bis-ANS. While bis-ANS significantly inhibited fibril formation 50 μM, did so relatively higher (400 μM). Alcohols, but not salts, were weaken inhibitory action these compounds suggesting possible involvement hydrophobic interactions their binding Besides, isothermal titration calorimetry molecular docking studies suggested that naphthalene derivatives mediated just through forces, disruption π–π between aromatic residues together inter-polypeptide chain repulsion among negatively charged ANS/bis-ANS bound SB.
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